Article
Interfacing Sca-1(pos) mesenchymal stem cells with biocompatible scaffolds with different chemical composition and geometry.
Journal of biomedicine & biotechnology - 1 Jan 2009
Forte G, Franzese O, Pagliari S, Pagliari F, Di Francesco A M, Cossa P, Laudisi A, Fiaccavento R, Minieri M, Bonmassar E, Di Nardo P
Abstract excerpt
An immortalized murine mesenchymal stem cell line (mTERT-MSC) enriched for Lin(neg)/Sca-1(pos) fraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation. Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of...
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