Article
Temporal aspects of major viral transcript expression in Hep G2 cells transfected with cloned hepatitis B virus DNA: with emphasis on the X transcript.
Virology - 1 Dec 1991
Wu H L, Chen P J, Lin M H, Chen D S
Abstract excerpt
The major transcripts of hepatitis B virus (HBV) and the kinetics of their expression were studied in a transient expression system by transfecting partially duplicated copies of HBV genome into Hep G2 cells. By Northern blotting, six species of HBV-specific transcripts could be identified. They were the pregenomic (3.6 kb), the preS1 (2.6 kb), the preS2/S (2.2 kb), the X (0.8 kb), and two spliced (2.2 kb) RNAs,...
Topics
- Base Sequence
- Blotting, Northern
- Gene Expression
- Genes, Overlapping
- Hepatitis B virus
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Plasmids