Article
Proton motive force dissipation precludes interaction of microcin J25 with RNA polymerase, but enhances reactive oxygen species overproduction.
Biochimica et biophysica acta - 1 Oct 2009
Dupuy Fernando G, Chirou María V Niklison, de Arcuri Beatriz Fernández, Minahk Carlos J, Morero Roberto D
Abstract excerpt
BACKGROUND: Microcin J25 targets the RNA polymerase as well as bacterial membranes. Because there is scarce information on the relationship between the uptake and the activity, a fluorescent microcin J25-derivative was used to further characterize its mechanism of action. METHODS: MccJ25 I13K was labeled with FITC and its uptake by sensitive cells was assessed by fluorescence measurements from supernatants of...
Topics
- 2,4-Dinitrophenol
- Amino Acid Substitution
- Anti-Bacterial Agents
- Bacteriocins
- Cell Membrane
- DNA-Directed RNA Polymerases
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Microbial Sensitivity Tests
- Mutation
- Oxygen Consumption