Article
Genotypic analysis of families with lactate dehydrogenase A (M) deficiency by selective DNA amplification.
Human genetics - 1 Nov 1991
Maekawa M, Sudo K, Li S S, Kanno T
Abstract excerpt
Genomic DNA prepared from LDH-A-deficient whole blood was amplified by the polymerase chain reaction technique using two primers specific for the active human LDH-A gene. The amplified fragment was examined by direct agarose gel electrophoresis, and a deletion of 20 base pairs (bp) in exon 6 of t...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Deletion
- DNA
- Electrophoresis, Agar Gel
- Female
- Genotype
- Humans
- L-Lactate Dehydrogenase
- Male
- Molecular Sequence Data
- Pedigree
- Phenotype
- Polymerase Chain Reaction