Article
Analysis of genetic mutations in human lactate dehydrogenase-A(M) deficiency using DNA conformation polymorphism in combination with polyacrylamide gradient gel and silver staining.
Biochemical and biophysical research communications - 31 Oct 1991
Maekawa M, Sudo K, Li S S, Kanno T
Abstract excerpt
Human lactate dehydrogenase (LDH)-A mutant gene was analyzed by polymerase chain reaction - DNA conformation polymorphism (DCP). We used polyacrylamide gradient gel and silver staining procedures for DCP analysis and observed abnormal migration patterns in individuals heterozygous for LDH-A defic...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA
- Electrophoresis, Polyacrylamide Gel
- Exons
- Genetic Carrier Screening
- Humans
- Introns
- Isoenzymes
- L-Lactate Dehydrogenase
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Polymorphism, Genetic