Article
Recovery of E,E-farnesol from cultures of yeast erg9 mutants: extraction with polymeric beads and purification by normal-phase chromatography.
Biotechnology progress - 1 Jan 2000
Song Linsheng
Abstract excerpt
Saccharomyces cerevisiae erg9 mutants blocked at squalene synthase require ergosterol for growth and produce E,E-farnesol. Typically, at least half the amount of farnesol remains cell associated. Practically insoluble in water, farnesol can be extracted from production cultures of the erg9 mutants using either methanol/hexane or poly(styrene-co-divinylbenzene) beads. The first method consumes more solvents and...
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