Article
Next-generation tag sequencing for cancer gene expression profiling.
Genome research - 1 Oct 2009
Morrissy A Sorana, Morin Ryan D, Delaney Allen, Zeng Thomas, McDonald Helen, Jones Steven, Zhao Yongjun, Hirst Martin, Marra Marco A
Abstract excerpt
We describe a new method, Tag-seq, which employs ultra high-throughput sequencing of 21 base pair cDNA tags for sensitive and cost-effective gene expression profiling. We compared Tag-seq data to LongSAGE data and observed improved representation of several classes of rare transcripts, including transcription factors, antisense transcripts, and intronic sequences, the latter possibly representing novel exons or...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.