Article
Clinical application of cytokine-related gene polymorphism analysis using a newly developed DNA chip in critically ill patients.
Clinical biochemistry - 1 Sept 2009
Otani Shunsuke, Oda Shigeto, Sadahiro Tomohito, Nakamura Masataka, Watanabe Eizo, Nakada Taka-aki, Abe Ryuzo, Tokuhisa Takeshi, Hirasawa Hiroyuki
Abstract excerpt
OBJECTIVES: To investigate the usefulness of analysis of single nucleotide polymorphism (SNP) using a newly developed DNA chip assay involving single base extension(SBE) and subsequent hybridization in cytokine-related genes in critical care patients. DESIGN AND METHODS: Genotyping was performed in 76 ICU patients admitted to the ICU. First, the DNA samples from 58 patients were subjected to PCR and SBE...
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