Directed evolution of ionizing radiation resistance in Escherichia coli.

doi:10.1128/JB.00502-09

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Article

Directed evolution of ionizing radiation resistance in Escherichia coli.

Journal of bacteriology - 1 Aug 2009

Harris Dennis R, Pollock Steve V, Wood Elizabeth A, Goiffon Reece J, Klingele Audrey J, Cabot Eric L, Schackwitz Wendy, Martin Joel, Eggington Julie, Durfee Timothy J, Middle Christina M, Norton Jason E, Popelars Michael C, Li Hao, Klugman Sarit A, Hamilton Lindsay L, Bane Lukas B, Pennacchio Len A, Albert Thomas J, Perna Nicole T, Cox Michael M, Battista John R

Abstract excerpt

We have generated extreme ionizing radiation resistance in a relatively sensitive bacterial species, Escherichia coli, by directed evolution. Four populations of Escherichia coli K-12 were derived independently from strain MG1655, with each specifically adapted to survive exposure to high doses of ionizing radiation. D(37) values for strains isolated from two of the populations approached that exhibited by...

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Identifiers

PMID: 19502398
DOI: 10.1128/JB.00502-09
PMCID: PMC2725583

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