Article
Zinc-finger directed double-strand breaks within CAG repeat tracts promote repeat instability in human cells.
Proceedings of the National Academy of Sciences of the United States of America - 16 Jun 2009
Mittelman David, Moye Christopher, Morton Jason, Sykoudis Kristen, Lin Yunfu, Carroll Dana, Wilson John H
Abstract excerpt
Expanded triplet repeats have been identified as the genetic basis for a growing number of neurological and skeletal disorders. To examine the contribution of double-strand break repair to CAG x CTG repeat instability in mammalian systems, we developed zinc finger nucleases (ZFNs) that recognize and cleave CAG repeat sequences. Engineered ZFNs use a tandem array of zinc fingers, fused to the FokI DNA cleavage...
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