Article
Immunolocalization of membrane-associated CTP:phosphocholine cytidylyltransferase in phosphatidylcholine-deficient Chinese hamster ovary cells.
The Journal of biological chemistry - 15 Mar 1992
Watkins J D, Kent C
Abstract excerpt
The location of CTP:phosphocholine cytidylyltransferase in Chinese hamster ovary (CHO) cells made deficient in phosphatidylcholine was determined by immunofluorescence techniques. A rabbit polyclonal antibody was raised against a synthetic peptide corresponding to the amino-terminal 17 amino acid residues of rat liver cytidylyltransferase. The antibody recognized both native and denatured cytidylyltransferase...
Topics
- Amino Acid Sequence
- Animals
- Antibodies
- Blotting, Western
- CHO Cells
- Cell Membrane
- Choline-Phosphate Cytidylyltransferase
- Cricetinae
- Endoplasmic Reticulum
- Fluorescent Antibody Technique
- Genetic Variation
