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Article

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.

PloS one - 1 Jan 2009

Engler Carola, Gruetzner Ramona, Kandzia Romy, Marillonnet Sylvestre

Abstract excerpt

We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of type IIs restriction enzymes and is performed by simply subjecting a mix of 10 undigested input plasmids (nine insert plasmids and the acceptor vector) to a...

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Source

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Identifiers

PMID: 19436741
DOI: 10.1371/journal.pone.0005553
PMCID: PMC2677662

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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