Article
Effects of mutations at Gly114 on the stability and refolding of Haloarchaeal nucleoside diphosphate kinase in low salt solution.
International journal of biological macromolecules - 1 May 2009
Ishibashi Matsujiro, Iwasa Tatsuya, Kumeda Kouko, Arakawa Tsutomu, Tokunaga Masao
Abstract excerpt
We have shown before that mutation of Gly114 to Arg enhances folding of hexameric nucleoside diphosphate kinase (HsNDK) from Halobacterium salinarum. In this study, we constructed three mutant forms, Gly114Lys (G114K), Gly114Ser (G114S) and Gly114Asp (G114D), to further clarify the role residue 114 plays in the stability and folding of HsNDK. While expression of G114D mutant resulted in inactive enzyme, other...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.