Article
Origins of the Intermediate Spectral Form in M100 Mutants of Photoactive Yellow Protein.
Photochemistry and photobiology - 1 Jan 2000
Kumar Anil, Woolley George Andrew
Abstract excerpt
Numerous single-site mutants of photoactive yellow protein (PYP) from Halorhodospira halophila and as well as PYP homologs from other species exhibit a shoulder on the short wavelength side of the absorbance maximum in their dark-adapted states. The structural basis for the occurrence of this shoulder, called the "intermediate spectral form," has only been investigated in detail for the Y42F mutation. Here we...
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