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Article

Increase of salt dependence of halophilic nucleoside diphosphate kinase caused by a single amino acid substitution.

Extremophiles : life under extreme conditions - 1 Jul 2013

Ishibashi Matsujiro, Hayashi Tomoe, Yoshida Chiho, Tokunaga Masao

Abstract excerpt

Nucleoside diphosphate kinase (HsNDK) from an extremely halophilic archaea, Halobacterium salinarum, is composed of a homo hexamer, assembled as a trimer of basic dimeric units. It requires >2 M NaCl for refolding, although it does not require NaCl for stability or enzymatic activity below 30 °C. A HisN111L mutant with an N-terminal extension sequence containing hexa-His tag, in which Asn111 was replaced with...

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Identifiers

PMID: 23609188
DOI: 10.1007/s00792-013-0541-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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