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Article

Reduction of salt-requirement of halophilic nucleoside diphosphate kinase by engineering S-S bond.

Archives of biochemistry and biophysics - 1 Sept 2012

Ishibashi Matsujiro, Uchino Manami, Arai Shigeki, Kuroki Ryota, Arakawa Tsutomu, Tokunaga Masao

Abstract excerpt

Nucleoside diphosphate kinase (HsNDK) from extremely halophilic haloarchaeon, Halobacterium salinarum, requires salt at high concentrations for folding. A D148C mutant, in which Asp148 was replaced with Cys, was designed to enhance stability and folding in low salt solution by S-S bond. It showed...

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Identifiers

PMID: 22683473
DOI: 10.1016/j.abb.2012.05.021

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-22683473-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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