Systematic mutation of bacteriophage T4 lysozyme.

Abstract excerpt

Amber mutations were introduced into every codon (except the initiating AUG) of the bacteriophage T4 lysozyme gene. The amber alleles were introduced into a bacteriophage P22 hybrid, called P22 e416, in which the normal P22 lysozyme gene is replaced by its T4 homologue, and which consequently depends upon T4 lysozyme for its ability to form a plaque. The resulting amber mutants were tested for plaque formation on...

Topics

• Amino Acid Sequence
• Base Sequence
• DNA, Viral
• Models, Molecular
• Molecular Sequence Data
• Muramidase
• Mutagenesis
• Phenotype
• Plasmids
• Proline
• Suppression, Genetic
• T-Phages