Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis.

doi:10.1021/bi00243a015

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Article

Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis.

Biochemistry - 23 Jul 1991

Sun D P, Sauer U, Nicholson H, Matthews B W

Abstract excerpt

Six designed mutants of T4 lysozyme were created in an attempt to create putative salt bridges on the surface of the protein. The first three of the mutants, T115E (Thr 115 to Glu), Q123E, and N144E, were designed to introduce a new charged side chain close to one or more existing charged groups...

Topics

Electrochemistry
Hydrogen-Ion Concentration
Models, Molecular
Muramidase
Mutagenesis, Site-Directed
Mutation
Protein Conformation
Protein Engineering
Salts
X-Ray Diffraction

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