Article
Direct visualisation and kinetic analysis of normal and nemaline myopathy actin polymerisation using total internal reflection microscopy.
Journal of muscle research and cell motility - 1 Jan 2009
Feng Juan-Juan, Ushakov Dmitry S, Ferenczi Michael A, Laing Nigel G, Nowak Kristen J, Marston Steven B
Abstract excerpt
Actin filaments were formed by elongation of pre-formed nuclei (short crosslinked actin-HMM complexes) that were attached to a microscope cover glass. By using TIRF illumination we could see actin filaments at high contrast despite the presence of 150 nM TRITC-phalloidin in the solution. Actin filaments showed rapid bending and translational movements due to Brownian motion but the presence of the methylcellulose...
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