Characterization of a mutation affecting the function of Escherichia coli folylpolyglutamate synthetase-dihydrofolate synthetase and further mutations produced in vitro at the same locus.

Abstract excerpt

The folC gene from mutant strain SF4 was cloned into a pUC19 plasmid. Expression of the mutant gene from the lac promoter of the plasmid complemented the auxotrophy for methionine of the SF4 strain. The only difference in sequence between the mutant and wild-type genes was a G925A base change resulting in an A309T amino acid change. The mutant enzyme had a 30-fold higher Km for 10-formyltetrahydrofolate as well...

Topics

• Escherichia coli
• Escherichia coli Proteins
• Genetic Complementation Test
• Kinetics
• Multienzyme Complexes
• Mutagenesis, Site-Directed
• Mutation
• Peptide Synthases
• Plasmids