Article
Novel mutations in the pheA gene of Escherichia coli K-12 which result in highly feedback inhibition-resistant variants of chorismate mutase/prephenate dehydratase.
Applied and environmental microbiology - 1 Aug 1992
Nelms J, Edwards R M, Warwick J, Fotheringham I
Abstract excerpt
The bifunctional enzyme chorismate mutase/prephenate dehydratase (EC 5.4.99.5/4.2.1.51), which is encoded by the pheA gene of Escherichia coli K-12, is subject to strong feedback inhibition by L-phenylalanine. Inhibition of the prephenate dehydratase activity is almost complete at concentrations of L-phenylalanine greater than 1 mM. The pheA gene was cloned, and the promoter region was modified to enable...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Feedback
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Prephenate Dehydratase
- Promoter Regions, Genetic