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Article

Transformation of Vibrio cholerae by plasmid DNA.

Gene - 30 Aug 1991

Panda D K, Dasgupta U, Das J

Abstract excerpt

The lack of an efficient transformation system in Vibrio cholerae was a handicap in the genetic manipulation of this important human pathogen. Since V. cholerae cells secrete DNases, this may interfere with the uptake of DNA. The present report describes the approaches taken for transforming V. cholerae cells with plasmid DNA, by overcoming this DNase barrier. The partial success of transforming DNase-negative...

Topics

Calcium
Deoxyribonucleases
Kinetics
Magnesium
Mutation
Plasmids
Temperature
Transformation, Bacterial
Vibrio cholerae

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1936999
DOI: 10.1016/0378-1119(91)90520-l

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1936999-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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