Transduction-like gene transfer in the methanogen Methanococcus voltae.

doi:10.1128/JB.181.10.2992-3002.1999

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Article

Transduction-like gene transfer in the methanogen Methanococcus voltae.

Journal of bacteriology - 1 May 1999

Bertani G

Abstract excerpt

Strain PS of Methanococcus voltae (a methanogenic, anaerobic archaebacterium) was shown to generate spontaneously 4.4-kbp chromosomal DNA fragments that are fully protected from DNase and that, upon contact with a cell, transform it genetically. This activity, here called VTA (voltae transfer agent), affects all markers tested: three different auxotrophies (histidine, purine, and cobalamin) and resistance to BES...

Topics

Air
Centrifugation, Isopycnic
Culture Media, Conditioned
DNA Restriction Enzymes
DNA, Archaeal
Deoxyribonucleases
Electrophoresis, Agar Gel
Gene Transfer Techniques
Genes, Archaeal
Genetic Markers
Genome
Kinetics
Methanococcus
Micropore Filters
Molecular Weight
Mutation
Recombination, Genetic
Spectrophotometry, Ultraviolet

Source

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Identifiers

PMID: 10321998
DOI: 10.1128/JB.181.10.2992-3002.1999
PMCID: PMC93752

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