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Article

Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its role in preventing uptake of foreign DNA.

Applied and environmental microbiology - 1 Jan 2001

Wu S I, Lo S K, Shao C P, Tsai H W, Hor L I

Abstract excerpt

We have cloned a nuclease gene, vvn, from Vibrio vulnificus, an estuarine bacterium that causes wound infections and septicemia in humans and eels. The gene contained a 696-bp open reading frame encoding 232 amino acids (aa), including a signal sequence of 18 aa. The deduced amino acid sequence of the mature nuclease predicted a molecular mass of 25 kDa, which was confirmed by vital stain, and a pI of 8.6. Vvn...

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Identifiers

PMID: 11133431
DOI: 10.1128/AEM.67.1.82-88.2001
PMCID: PMC92521

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