Optimization of Yarrowia lipolytica-based consolidated biocatalyst through synthetic biology approach: transcription units and signal peptides shuffling.

Abstract excerpt

Nowadays considerable effort is being pursued towards development of consolidated microbial biocatalysts that will be able to utilize complex, non-pretreated substrates and produce valuable compounds. In such engineered microbes, synthesis of extracellular hydrolases may be fine-tuned by different approaches, like strength of promoter, type of secretory tag, and gene copy number. In this study, we investigated if...

Topics

• Biocatalysis
• Bioreactors
• Fungal Proteins
• Gene Dosage
• Gene Expression
• Lipids
• Phenotype
• Promoter Regions, Genetic
• Protein Sorting Signals
• Recombinant Proteins