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Article

Enzymatic synthesis of c-di-GMP using a thermophilic diguanylate cyclase.

Analytical biochemistry - 15 Jun 2009

Rao Feng, Pasunooti Swathi, Ng Yinglu, Zhuo Weichao, Lim Lishi, Liu Angeline Weixian, Liang Zhao-Xun

Abstract excerpt

The cyclic dinucleotide c-di-GMP is a widespread bacterial messenger molecule with potential application as a therapeutic agent for treating bacterial infection. Current enzymatic synthesis of c-di-GMP using mesophilic diguanylate cyclase (DGC) proteins suffers from low production yield due to protein instability and strong product inhibition. Here we report the overexpression and characterization of a...

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Identifiers

PMID: 19328769
DOI: 10.1016/j.ab.2009.03.031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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