Article
Fluorescence study of a mutant cytochrome b5 with a single tryptophan in the membrane-binding domain.
Biochemistry - 22 Oct 1991
Ladokhin A S, Wang L, Steggles A W, Holloway P W
Abstract excerpt
Fluorescence studies of cytochrome b5 are complicated by the presence of three tryptophans, at positions 108, 109, and 112, in the membrane-binding domain. The cDNA for rabbit liver cytochrome b5, isolated from a lambda gt11 library, was used to generate a mutated mRNA where the codons for trypto...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cell Membrane
- Cytochromes b5
- Lipids
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Octoxynol
- Polyethylene Glycols