Article
Mutation analysis of the interaction of B-type cytochrome c oxidase with its natural substrate cytochrome c-551.
Journal of bioscience and bioengineering - 1 Apr 2010
Kabashima Yoshiki, Ueda Naoaki, Sone Nobuhito, Sakamoto Junshi
Abstract excerpt
Heme-copper oxidases in the respiratory chain are classified into three subfamilies: A-, B- and C-types. Cytochrome bo(3)-type cytochrome c oxidase from thermophilic Bacillus is a B-type oxidase that is thought to interact with cytochrome c through hydrophobic interactions. This is in contrast to A-type oxidases, which bind cytochrome c molecules primarily through electrostatic forces between acidic residues in...
Topics
- Amino Acid Sequence
- Bacillus
- Bacterial Proteins
- Base Sequence
- Catalytic Domain
- Cytochrome c Group
- DNA Primers
- DNA, Bacterial
- Electron Transport Complex IV
- Genes, Bacterial
- Geobacillus
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Substrate Specificity