Article
The expression of bovine microsomal cytochrome b5 in Escherichia coli and a study of the solution structure and stability of variant proteins.
Protein engineering - 1 Nov 1993
Hewson R, Newbold R J, Whitford D
Abstract excerpt
The DNA sequence of bovine microsomal cytochrome b5 has been amplified from a liver cDNA library using a polymerase chain reaction. The amplified cDNA when cloned into plasmids that support the high-level production of cytochrome b5 in E.coli leads to protein overexpression and results in cell co...
Topics
- Animals
- Cattle
- Cell Compartmentation
- Cloning, Molecular
- Cytochromes b5
- Cytoplasm
- DNA, Complementary
- Escherichia coli
- Genetic Variation
- Magnetic Resonance Spectroscopy
- Membranes
- Microsomes
- Mutagenesis
- Protein Denaturation
- Recombinant Proteins
- Sequence Analysis, DNA
- Sequence Deletion