Article
Expression of cytochrome b5 in yeast and characterization of mutants of the membrane-anchoring domain.
The Journal of biological chemistry - 25 Jun 1992
Vergères G, Waskell L
Abstract excerpt
The soluble and membrane-bound forms of the synthetic rat cytochrome b5 gene have been expressed in Saccharomyces cerevisiae. In order to examine the topology and function of the COOH-terminal membrane binding domain of cytochrome b5, mutants have been constructed, expressed, purified, and partia...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cytochromes b5
- DNA
- Heme
- Hydrogen-Ion Concentration
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Peptides
- Protein Conformation
- Rats
- Saccharomyces cerevisiae
- Solubility