Article
Detection of K-Ras mutations in tumour samples of patients with non-small cell lung cancer using PNA-mediated PCR clamping.
British journal of cancer - 24 Mar 2009
Beau-Faller M, Legrain M, Voegeli A-C, Guérin E, Lavaux T, Ruppert A-M, Neuville A, Massard G, Wihlm J-M, Quoix E, Oudet P, Gaub M P
Abstract excerpt
Non-small cell lung cancers (NSCLC), in particular adenocarcinoma, are often mixed with normal cells. Therefore, low sensitivity of direct sequencing used for K-Ras mutation analysis could be inadequate in some cases. Our study focused on the possibility to increase the detection of K-Ras mutations in cases of low tumour cellularity. Besides direct sequencing, we used wild-type hybridisation probes and...
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