Article
Solution structure and backbone dynamics of streptopain: insight into diverse substrate specificity.
The Journal of biological chemistry - 17 Apr 2009
Wang Chih-Chieh, Houng Hsiang-Chee, Chen Chun-Liang, Wang Pei-Ju, Kuo Chih-Feng, Lin Yee-Shin, Wu Jiunn-Jong, Lin Ming T, Liu Ching-Chuan, Huang Wenya, Chuang Woei-Jer
Abstract excerpt
Streptococcal pyrogenic exotoxin B (SPE B) is a cysteine protease expressed by Streptococcus pyogenes. The D9N, G163S, G163S/A172S, and G239D mutant proteins were expressed to study the effect of the allelic variants on their protease activity. In contrast to other mutants, the G239D mutant was approximately 12-fold less active. The Gly-239 residue is located within the C-terminal S230-G239 region, which cannot...
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