Article
[Establishment of a semi-nested PCR for identifying the sub-genotypes (Ba and Bj) of hepatitis B virus of genotype B].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Feb 2008
Wang Jie, Gao Jun-Wei, Li Jie, Zhuang Hui, Wang Jia, Li Ya-Juan, Jin Hui
Abstract excerpt
OBJECTIVE: To establish a sensitive, specific, simple and practicable method for identifying the two sub-genotypes (Ba and Bj) of genotype B isolates of hepatitis B virus (HBV) (HBV/ B). METHODS: The entire nucleotide sequences of HBV/B and HBV/C were obtained from GenBank, compared and analyzed with DNAStar software. The specific primers for HBV/B (HB) and Ba (BA), Bj (BJ) were designed respectively. HB as HBV/B...
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