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Article

A twin-reporter vector for simultaneous analysis of expression signals of divergently transcribed, contiguous genes in filamentous fungi.

Gene - 31 Jul 1991

Punt P J, Greaves P A, Kuyvenhoven A, van Deutekom J C, Kinghorn J R, Pouwels P H, van den Hondel C A

Abstract excerpt

To analyze the promoter region(s) of divergently transcribed fungal genes, a twin-reporter vector was constructed. This vector contains two divergently oriented reported genes, encoding Escherichia coli beta-glucuronidase (uidA) and E. coli beta-galactosidase (lacZ). Terminator regions of the Aspergillus nidulans nitrate and nitrite reductase-encoding genes, niaD and niiA, respectively, have been cloned 3' to the...

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PMID: 1916271
DOI: 10.1016/0378-1119(91)90476-r

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