Article
Functional analysis of the expression of the 3'-phosphoglycerate kinase pgk gene in Aspergillus nidulans.
Molecular & general genetics : MGG - 1 May 1992
Streatfield S J, Toews S, Roberts C F
Abstract excerpt
A functional analysis of the Aspergillus nidulans 3-phosphoglycerate kinase pgk promoter was undertaken using gene fusions to the lacZ gene of Escherichia coli, and introducing these into a beta-galactosidase-deficient strain of A. nidulans. Expression of a particular gene fusion in transformed strains depends upon the site of integration of the vector into the genome, and when specifically targeted to the...
Topics
- Aspergillus nidulans
- Cloning, Molecular
- DNA, Fungal
- Electrophoresis, Agar Gel
- Escherichia coli
- Gene Expression
- Mutation
- Phosphoglycerate Kinase
- Promoter Regions, Genetic
- RNA, Fungal
- Recombinant Fusion Proteins
