Article
Molecular cloning and functional characterization of the pathway-specific regulatory gene nirA, which controls nitrate assimilation in Aspergillus nidulans.
Molecular and cellular biology - 1 Feb 1991
Burger G, Tilburn J, Scazzocchio C
Abstract excerpt
We have cloned an 11-kbp segment of the genomic DNA of Aspergillus nidulans which complements mutations in nirA, the pathway-specific regulatory gene of the nitrate assimilation pathway. Gene disruption in the corresponding region of the nuclear DNA leads to a phenotype and a gene complementation pattern indistinguishable from that observed in known noninducible nirA mutants. Transformation studies with subclones...
Topics
- Aspergillus nidulans
- Cloning, Molecular
- Crosses, Genetic
- DNA, Fungal
- Genes, Fungal
- Genes, Regulator
- Genetic Linkage
- Genomic Library
- Genotype
- Nitrates
- Plasmids