Article
Towards a transgenic mouse model of sickle cell disease: hemoglobin SAD.
The EMBO journal - 1 Nov 1991
Trudel M, Saadane N, Garel M C, Bardakdjian-Michau J, Blouquit Y, Guerquin-Kern J L, Rouyer-Fessard P, Vidaud D, Pachnis A, Roméo P H
Abstract excerpt
In order to obtain a transgenic mouse model of sickle cell disease, we have synthesized a novel human beta-globin gene, beta SAD, designed to increase the polymerization of the transgenic human hemoglobin S (Hb S) in vivo. beta SAD (beta S-Antilles-D Punjab) includes the beta 6Val substitution of...
Topics
- Anemia, Sickle Cell
- Animals
- Chromatography, High Pressure Liquid
- DNA
- Disease Models, Animal
- Electrophoresis, Polyacrylamide Gel
- Erythrocyte Indices
- Globins
- Hemoglobin, Sickle
- Isoelectric Focusing
- Mice
- Mice, Transgenic
- Oxygen
- Peptide Mapping
- Phenotype