Article
Cycloheximide resistance as a yeast cloning marker.
Current genetics - 1 May 1991
del Pozo L, Abarca D, Claros M G, Jiménez A
Abstract excerpt
In CYH2/cyh2 heterozygous diploids of the yeast Saccharomyces cerevisiae resistance is dominant over sensitivity at low (0.5-5 micrograms/ml) cycloheximide (cyh) concentrations. The cyh-resistant haploid strain MMY1 confers relatively high (10 micrograms/ml) cyh-resistance to heterozygous diploids constructed by mating this strain with cyh-sensitive haploid strains. We present here a genetic and biochemical study...
Topics
- Chromosome Mapping
- Chromosomes, Fungal
- Cloning, Molecular
- Cycloheximide
- Diploidy
- Drug Resistance, Microbial
- Genetic Markers
- Heterozygote
- Phenotype
- Restriction Mapping
- Saccharomyces cerevisiae
