Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces.

Genetics - 1 May 1990

Leppert G, McDevitt R, Falco S C, Van Dyk T K, Ficke M B, Golin J

Abstract excerpt

Yeast DNA fragments that confer multiple drug resistance when amplified were isolated. Cells containing a yeast genomic library cloned in the high copy autonomously replicating vector, YEp24, were plated on medium containing cycloheximide. Five out of 100 cycloheximide-resistant colonies were cross-resistant to the unrelated inhibitor, sulfometuron methyl, due to a plasmid-borne resistance determinant. The...

Topics

Acetolactate Synthase
Chromosome Mapping
Cloning, Molecular
Cycloheximide
DNA Transposable Elements
Drug Resistance, Microbial
Gene Amplification
Genes, Fungal
Genetic Linkage
Phenotype
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Saccharomyces cerevisiae

Sulfonylurea Compounds

Transformation, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2160400
DOI: 10.1093/genetics/125.1.13
PMCID: PMC1203995

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2160400-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces.PMID 2160400Read ↗

Multiple or pleiotropic drug resistance in yeast.Semantic proximity Read ↗

Interaction of the yeast pleiotropic drug resistance genes PDR1 and PDR5.Semantic proximity Read ↗

Mutations in the yeast PDR3, PDR4, PDR7 and PDR9 pleiotropic (multiple) drug resistance loci affect the transcript level of an ATP binding cassette transporter encoding gene, PDR5.Semantic proximity Read ↗

The unusual inheritance of multidrug-resistance factors in Saccharomyces.Semantic proximity Read ↗

Analysis of second-site mutations that suppress the multiple drug resistance phenotype of the yeast PDR1-7 allele.Semantic proximity Read ↗

Activation of the pleiotropic drug resistance pathway can promote mitochondrial DNA retention by fusion-defective mitochondria in Saccharomyces cerevisiae.Semantic proximity Read ↗

Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.Semantic proximity Read ↗

Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae.Semantic proximity Read ↗

Drug resistance in diploid yeast is acquired through dominant alleles, haploinsufficiency, gene duplication and aneuploidy.Semantic proximity Read ↗

Heterologous expression of a pleiotropic drug resistance transporter from Phytophthora sojae in yeast transporter mutants.Semantic proximity Read ↗

Diazaborine resistance in the yeast Saccharomyces cerevisiae reveals a link between YAP1 and the pleiotropic drug resistance genes PDR1 and PDR3.Semantic proximity Read ↗

The transporters Pdr5p and Snq2p mediate diazaborine resistance and are under the control of the gain-of-function allele PDR1-12.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.