Article
Long-term survival of Escherichia coli lacking the HipBA toxin-antitoxin system during prolonged cultivation.
Bioscience, biotechnology, and biochemistry - 1 Jan 2009
Kawano Hiroaki, Hirokawa Yasutaka, Mori Hideo
Abstract excerpt
We designed and constructed six major toxin-antitoxin disruptants (DeltachpBIK, DeltadinJ-yafQ, DeltahipBA, DeltamazEF, DeltarelBE, and DeltayefM-yoeB) of Escherichia coli K-12 W3110. On prolonged cultivation of these disruptants with minimal M9 medium, the DeltahipBA cells exhibited a significantly longer life span than that of the other disruptants and of wild-type cells, as analyzed with a LIVE/DEAD BacLight...
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