Article
Interaction of Sp1 with the human gamma globin promoter: binding and transactivation of normal and mutant promoters.
Blood - 1 Oct 1991
Gumucio D L, Rood K L, Blanchard-McQuate K L, Gray T A, Saulino A, Collins F S
Abstract excerpt
We have analyzed the binding of Sp1, a ubiquitously expressed transactivator, to the promoter region of the gamma genes. Low-affinity Sp1 sites were found at -50 and -200. A high-affinity site was detected at -140, over the CACCC sequence. To analyze the function of these sites, Drosophila SL-2 c...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Deoxyribonuclease I
- Drosophila
- Globins
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Promoter Regions, Genetic
- Sp1 Transcription Factor
- Transcriptional Activation
- Transfection