Article
Characterization of a GC-rich region containing Sp1 binding site(s) as a constitutive responsive element of the alpha 2(I) collagen gene in human fibroblasts.
The Journal of biological chemistry - 3 Mar 1995
Tamaki T, Ohnishi K, Hartl C, LeRoy E C, Trojanowska M
Abstract excerpt
To analyze regulatory elements in the human alpha 2(I) collagen gene (COL1A2) promoter, a series of deletion mutants from -323 to -186 base pairs was tested in transient transfection assays in human fibroblasts. A strong positive responsive element was mapped to a GC-rich region located between b...
Topics
- Base Sequence
- Binding Sites
- Cells, Cultured
- Collagen
- Cytosine
- DNA Probes
- Fibroblasts
- Guanine
- Humans
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Sp1 Transcription Factor
- Transcription, Genetic