Article
[A novel method of detecting mitochondrial C1494T and A1555G mutations by using the base-quenched probe technique in a single PCR assay].
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery - 1 Apr 2012
ZHENG Lu, LUO Guang-hua, ZHANG Jun, ZHANG Xiao-ying, XU Ning
Abstract excerpt
OBJECTIVE: In the present study we describe a new method to detect the mitochondrial DNA (mtDNA) mutations A1555G and C1494T by using the base-quenched probe technique in a single PCR reaction. METHODS: 6-carboxyfluorescein (FAM) was directly conjugated to the 3' end of the probe. Four vectors, representing the four possible genotype combinations, were constructed as the amplification template for the methodology...
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