Article
Augmented production of alginate oligosaccharides by the Pseudomonas mendocina NK-01 mutant.
Carbohydrate research - 1 May 2012
Guo Wenbin, Feng Jun, Geng Weitao, Song Cunjiang, Wang Yuanyuan, Chen Ning, Wang Shufang
Abstract excerpt
Pseudomonas mendocina NK-01 can simultaneously synthesize medium-chain-length polyhydroxyalkanoate (PHA(MCL)) and alginate oligosaccharides (AO) from glucose under conditions of limited nitrogen. In this study, the PHA(MCL) synthesis pathway was blocked by a deletion of approximately 57% of the sequence of PHA synthase operon mediated by the suicide plasmid, pEX18TcC1ZC2Amp. Deletion of the PHA synthase operon in...
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