Article
Genetic and ultrastructural analysis of different mutants of Pseudomonas putida affected in the poly-3-hydroxy-n-alkanoate gene cluster.
Environmental microbiology - 1 Mar 2007
Sandoval Angel, Arias-Barrau Elsa, Arcos Mario, Naharro Germán, Olivera Elías R, Luengo José M
Abstract excerpt
Functional analyses of the different proteins involved in the synthesis and accumulation of polyhydroxyalkanoates (PHAs) in P. putida U were performed using a mutant in which the pha locus had been deleted (PpUDeltapha). These studies showed that: (i) Pha enzymes cannot be replaced by other proteins in this bacterium, (ii) the transformation of PpDeltapha with a plasmid containing the locus pha fully restores the...
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