Article
Utilization of fadA knockout mutant Pseudomonas putida for overproduction of medium chain-length-polyhydroxyalkanoate.
Biotechnology letters - 1 Dec 2007
Vo Minh Tri, Lee Kwang-Woo, Kim Tae-Kwon, Lee Yong-Hyun
Abstract excerpt
The fadBA operon in the fatty acid beta-oxidation pathway of P. putida KCTC1639 was blocked to induce a metabolic flux of the intermediates to the biosynthesis of medium chain-length PHA (mcl-PHA). Succinate at 150 mg l(-1) stimulated cell growth and also the biosynthesis of medium chain-length-polyhydroxyalkanoate. pH-stat fed-batch cultivation of the fadA knockout mutant P. putida KCTC1639 was carried out for...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
