Article
Molecular analysis of lambda bio transducing phage produced by oxolinic acid-induced illegitimate recombination in vivo.
Genetics - 1 Jul 1995
Shimizu H, Yamaguchi H, Ikeda H
Abstract excerpt
To study the mechanism of DNA gyrase-mediated illegitimate recombination in Escherichia coli, we examined the formation of lambda Spi- phage during prophage induction. The frequency of Spi- phage was two to three orders of magnitude higher in the presence of oxolinic acid, an inhibitor of DNA gyrase A subunit, than in the absence of the drug, while it was very low in nalAr bacteria with the drug. RecA function is...
Topics
- Bacteriophage lambda
- Base Sequence
- DNA Topoisomerases, Type II
- DNA, Viral
- Escherichia coli
- Genes, Viral
- Genotype
- Molecular Sequence Data
- Oxolinic Acid
- Polymerase Chain Reaction
- Recombination, Genetic