Article
Functional complementation of yeast mutants to study plant signalling pathways.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Mehlmer Norbert, Scheikl-Pourkhalil Elisabeth, Teige Markus
Abstract excerpt
The rapidly increasing amount of entirely sequenced genomes generates a need for fast and efficient methods to elucidate gene functions. Functional complementation of yeast mutants, displaying selectable phenotypes, has been used very successfully in the past years to isolate many plant genes involved in signalling, stress response or metabolic pathways. Using the well-characterized Hog1 pathway, a mitogen...
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