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Article

Using directed evolution to probe the substrate specificity of mandelamide hydrolase.

Protein engineering, design & selection : PEDS - 1 Feb 2009

Wang Pan-Fen, Yep Alejandra, Kenyon George L, McLeish Michael J

Abstract excerpt

Mandelamide hydrolase (MAH), a member of the amidase signature family, catalyzes the hydrolysis of mandelamide to mandelate and ammonia. X-ray structures of several members of this family, but not that of MAH, have been reported. These reveal nearly superimposable conformations of the unusual Ser-cisSer-Lys catalytic triad. Conversely, the residues involved in substrate recognition are not conserved, implying...

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Identifiers

PMID: 19074156
DOI: 10.1093/protein/gzn073

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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