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Article

Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta.

Protein science : a publication of the Protein Society - 1 Jan 2002

Lauble Hanspeter, Miehlich Burkhard, Förster Siegfried, Kobler Christoph, Wajant Harald, Effenberger Franz

Abstract excerpt

Tryptophan 128 of hydroxynitrile lyase of Manihot esculenta (MeHNL) covers a significant part of a hydrophobic channel that gives access to the active site of the enzyme. This residue was therefore substituted in the mutant MeHNL-W128A by alanine to study its importance for the substrate specificity of the enzyme. Wild-type MeHNL and MeHNL-W128A showed comparable activity on the natural substrate acetone...

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Identifiers

PMID: 11742123
DOI: 10.1110/ps.33702
PMCID: PMC2368774

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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